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Description
Replacing asparagine with glutamine at the 8th position might potentially reduce the risk of asparagine rearrangement and amide hydrolysis

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After incubation, 150 L of the assay sample was added to 50 L of perchloric acid [final concentration 2.5% ( w / v )] to stop the reaction and spun down for 30 min prior transferring 80 L to an HPLC vial

Renal Physiol

VarelaLpez A., Bulln P., Giampieri F., and Quiles J