estimation of glutathione-s-transferase Frontiers A Novel High-Throughput Colorimetric Assay
Description
Fritz, Y

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The molecule contains a cysteine-linked disulfide bridge that constrains the peptide into a cyclic conformation, an important feature for maintaining its signaling profile in biochemical and adipocyte-based assays

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Even the angle at which you introduce water into the vial can determine whether you end up with fully functional GHK-Cu or an inactive solution of broken peptide fragments
