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Goat anti-Rabbit Rhodamine Red-X (1:500) was used a as a secondary antibody and Hoechst 34580 (1:500) was applied for 20 min to counterstain nuclei

In diet-induced obesity (DIO) models, NNMT loss-of-function and selective small-molecule inhibitors reduce white adipose mass and improve metabolic parameters without overt toxicity, positioning NNMT as a tractable target for metabolic-disease research [24]

The KD group maintained physiological ketosis

Adenosylcobalamin is needed as cofactor in methylmalonyl-CoA mutaseMUT enzyme

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Several analogues exhibit undesirable reactivity, especially toward glutathione, cysteine residues in proteins, and DNA, due to the dienone moiety, which limits their clinical applicability