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Western blotting Cell lysates were collected in RIPA buffer (150 mM NaCl, 1.0% NP-40, 0.5% sodium deoxycholate, 0.1% SDS (sodium dodecyl sulphate)and Western blotting was performed as previously described 49

Methodology: R.G., K.J., J.H., A.W., S.B., F.P., M.D., N.K., C.C., K.W., D.I., T.P.W., C.S., M.J., F.A.M., E.T.K., I.T., B.H.C., F.M., V.H., S.J., P.S., F.O., S.O.R., E.F.F

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The autophagy-inhibitor bafilomycin A1 (100 nM) and the autophagy-inducer rapamycin (10 M) were used as negative and positive controls, respectively

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10.1002/humu.21477 166 WuW.HuZ.QinY.DongJ.DaiJ.LuC.et al (2012)