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Other symptoms to look for include pain during urination, a sudden urge to urinate more frequently, or a persistent fever

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Membranes were pre-incubated in a 3% bovine serum albumin/Tris buffered saline with Tween 20 solution at room temperature for 30 min to block non-specific binding, followed by overnight incubation at 4 C with rabbit anti-rat interleukin (IL)-6 primary antibody (1:2000 dilution