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doi:10.1136/jech-2014-204971 [23] Cohen L, Jefferies A
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The pattern of induction of downstream effector protein kinases (Erk1/2 and Akt) was examined to further characterize the activity profile of small BDNF mimetic GSB-106 in serum starved cells
Once the lysate had been loaded, the column was washed with 20 column volumes (CV) wash buffer, and the His-tagged TRFH domain was eluted using a linear gradient from 0 to 100% elution buffer (50 mM Tris-HCl pH 8.0, 500 mM NaCl, 1 mM TCEP, 300 mM imidazole) over 25 CV
