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Reliable quantification of individual NAD metabolites and glutathione can be achieved through liquid-chromatography mass-spectrometry (LC-MS) 22 , cyclic enzymatic assays and high-performance liquid chromatography (HPLC) 2326 , but these have not been applicable for the scalable analysis of the whole redox set, especially from a single extract

Other forms of cell death were discovered later, such as pyroptosis and necrosis

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doi: 10.1083/jcb.105.2.1023 87

Thus, once MTHFR has reduced 5,10-methyleneTHF to 5-MTHF, the Vitamin B12 dependent methionine synthase, encoded by MTR, uses this reduced folate to convert Hcy back into methionine