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To gain a deeper understanding of the cellular mechanisms underlying WD, a reanalysis of the GSE1073236 dataset was conducted and validated by comparative analyses in a cell culture model of HepG2 and HepG2 ATP7B knockout (ATP7B-KO) cells, as well as in primary hepatocytes from WD patients and controls

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This activates the PI3K/AKT pathway, which promotes angiogenesis and expands the neurovascular niche [12]

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Then these libraries were sequenced on the Illumina sequencing platform HiSeqTM 2500 and 125 bp/150 bp paired-end reads were generated