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A high GSH:GSSG ratio signals strong cellular health and acts as a biomarker for oxidative stress

coli (OD 600 0.5) were washed twice with sodium phosphate buffer (100 mM Na-phosphate buffer (Na 2 HPO 4 /NaH 2 PO 4 ), 0.5 mM EDTA, pH 7.5) and lysed using a triple freezing procedure (liquid nitrogen, 196C), defrosting (37C)

Main Bioenergetic and biosynthetic processes are necessary for tissue growth and functioning and rely on appropriate oxygen and nutrient supply

The Search tool in the KEGG mapper 159 was used to aid in integrated analysis of DAPs and differentially abundant metabolites

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PMID: 22532519