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In the course of the experiments, tert-butyl hydroperoxide (TBHP) was used to induce oxidative stress in annulus fibrosus cells (AFCs) and nucleus pulposus cells (NPCs)

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It is commonly used in dietary supplements due to its vital role in maintaining cellular structure and function

When ROS levels exceed the antioxidant defenses, spermatozoa are subjected to oxidative stress, leading to detrimental molecular changes, including lipid peroxidation, DNA fragmentation, and protein oxidation

subhirsutum leaves were freshly collected from young seedling plants and washed with sterile distilled to eliminate debris

Vachharajani V, McCall CE