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El primer enzim en aquesta via, la glioxalasa I, catalitza la isomeritzaci dels adductes hemiacetals, que sn formats en una reacci espontnia entre un glutati i aldehids, com el metilglioxal: glutati + metilglioxal adducte hemiacetal adducte hemiacetal + glioxalasa I S-lactoilglutati El segon enzim, la glioxalasa II, catalitza la hidrlisi del producte de la reacci anterior, el S-lactoilglutati: S-lactoil-GSH + HO D-cid lctic + GSH El GSH tamb pot estar implicat en la detoxificaci de formaldehid produt de forma endgena