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Briefly, 50 ug total RNA was diluted with H 2 O in 100 ul volume and then denatured under 65 C for 2 min, and immediately put on the ice for exactly 2 min

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pgl-1(q994 )[PGL-1::SNAP::N22] /nT1[qIs51](IV;V) JK6148: wago-1(q1089) [WAGO-1(null deletion)::3xV5]