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The cells were washed with PBS and incubated in fresh medium for 9 h

Reactive oxygen species production by monoamine oxidases in intact cells

The cystathionine -lyase-mediated persulfidation of cGAPDH directly contributes to the increased production of NAD + via the reduction of glyceraldehyde-3-phosphate to glycerol-3-phosphate and the oxidation of NADH to NAD +
Second, mitochondrial energy sensing functions have bidirectional effects on ferroptosis: AMPK regulates ferroptosis through ACC phosphorylation and inactivation, while also inhibiting ferroptosis by suppressing fatty acid biosynthesis [29, 30]
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