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Vitamin B5 (d-calcium Pantothenate): Vitamin B5 is essential for the breakdown of fatty acids, amino acids, steroids, and cholesterol

Your tagged protein of interest will bind to the column while other proteins flow through

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Folate must be checked alongside B12, and folate deficiency should never be treated in isolation when B12 is low, because doing so can mask the anaemia while neurological damage progresses