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Analogy to progressive somatic mutations in cancer evolution In cancer development and progression (for colon, breast, and lung malignancies), there appears to be an accumulation of a series of somatic mutations, typically involving loss of protective oncogenes or tumor suppressive factors and increases in cellular proliferative factors

Mobile phase A consisted of 98% H 2 O and 2% acetonitrile with 0.1% formic acid, while mobile phase B comprised 98% acetonitrile and 2% H 2 O with 0.1% formic acid

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Furthermore, women with endometriosis have a marked increase in TNF- concentration in the granulosa cells [103, 104, 105, 106], which leads to the reduction in the size of the inner cell mass and in the proliferation of the trophoectoderm in the blastocyst, it was observed that the integration of the culture medium with LC allowed to neutralize the antiproliferative effect of TNF- and to limit DNA damage during embryo development [93]