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Gclm KO mice also exhibit constitutive activation of liver AMP-activated protein kinase (AMPK) pathway and nuclear factor-erythroid 2related factor 2 target genes, and show enhanced ethanol clearance, altered hepatic lipid profiles in favor of increased levels of polyunsaturated fatty acids and concordant changes in expression of genes associated with lipogenesis and fatty acid oxidation

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A: Generally yes, but spacing is recommended

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Left gel shows very little GST-fusion protein in the flow-through and a lot in the elution indicating that sufficient glutathione agarose resin was used in this purification