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Samples were centrifuged for 10 min at 200 g at 4C, the supernatant was removed, and the cell pellet was resuspended in 2% BSA in PBS and the suspension was centrifuged for 10 min at 200 g at 4C

Diabetic hyperglycemia further exacerbates this by suppressing astrocytic pentose phosphate pathway flux, which depletes nicotinamide adenine dinucleotide phosphate (NADPH) reserves [132, 133], a mechanism that mirrors defects in diabetic neuropathy where impaired redox defense amplifies oxidative injury

International Journal of Andrology 16 18318

Younes I, Singh Z, Hammad W, Mowafy A, Remolina C

5133, Oct

No, not on its own
