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To view a copy of this licence, visit About this article Cite this article Hsieh, MJ., Lee, CH., Chueh, HY

Uysal F, Akkoyunlu G, Ozturk S

Writing review & editing: RR, SB, TPK

Transmission electron microscopy The fixed organoids (described above) were rinsed and incubated overnight in 10% sucrose (in water) at 4 C, and the organoids were dehydrated in graded alcohols (50%, 75%, 96%, 100%), cleared in propylene oxide and embedded in a mixture of Epon 812 and Durcupan (Sigma
IV therapy bypasses digestion and increases circulating levels temporarily
These in vitro results demonstrated that H2O2 induces Leydig cell senescence, accompanied by FOXO4 nuclear translocation and activation of senescence pathways
