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There, and everywhere: the wide host range and geographic distribution of zoonotic orthopoxviruses

S2(R1) recommends two options for such assessment

Approximately 100 mg of frozen tissue was minced in 500 l ice-cold homogenization buffer and homogenized in a Dounce homogenizer

The polymorphic cytochrome P-4502D6 is involved in the metabolism of both 5-hydroxytryptamine antagonists, tropisetron and ondansetron

Hutchinson AJ, Chou CL, Israel DD, Xu W, Regan JW (2009) Activation of EP2 prostanoid receptors in human glial cell lines stimulates the secretion of BDNF

For the identification of druggable targets, we considered three classes of enzymes: tier-1 targets, tier-2 targets, and other enzymes. Tier-1 targets correspond to enzymes whose knockout impedes viral replication while having a minimal impact on the simulated cellular viability