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First, fragmentation and ligation of adapters were performed using the commercial MGIEasy Fast FS Library Prep Module (MGI, China) and MGIEasy UDB Adapter Kit (MGI, China) according to the manufacturers protocol

doi: 10.1210/jc.2010-2905 49 FallahiPFerrariSMRagusaFRuffilliIEliaGPaparoSRet al

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After a further centrifugation (200 x g, 8 min, 20C, Universal 320, Andreas Hettich GmbH, Tuttlingen, Germany), PBMC were resuspended in RPMI medium supplemented with fetal bovine serum, HEPES buffer, L-glutamine, and -mercaptoethanol (all purchased from Biochrom AG, Berlin, Germany)