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The area covered by blood vessels was determined from the collagen 1 staining, not from a specific vessel marker such as VEGF or CD31

Therefore, the focus of assessment and treatment is not exclusively cortisol but rather the entire HPA axis

Any deficiency will contribute to fatigue, poor appetite, depression, and numbness/tingling in the hands and/or feet

The obtained tissue explants were cultured in DMEM/F-12 (Gibco, USA) supplemented with 10% fetal bovine serum (FBS, Gibco, USA) and 2% penicillin/streptomycin (Sigma, Germany) and incubated at 37 C and 5% CO 2 until the outgrowing cell confluence reached 80%

Injections of B12 are useful in such cases

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