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Results NF1 was frequently mutated and associated with poor prognosis in GBC To investigate the potential role of NF1 in GBC, a comprehensive analysis of NF1 expression in the tumor spectrum was conducted through the TCGA and GTEx databases

Participants received 600 mg of benfotiamine or placebo, once a day for 24 weeks

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After 60 min blocking with 5% dry milk (Sigma-Aldrich) in Tris-buffered saline with 0.1% Tween-20, membranes were incubated overnight at 4 C with primary antibodies detecting ATF4 (11815, Cell Signaling Technology, 1:1,000), ATF6 (70B1413.1, Novus Biologicals, 1:1,000), -actin (A5441, Sigma-Aldrich, 1:10,000), eIF2a (5324, Cell Signaling Technology, 1:1,000), G6PDH (8866, Cell Signaling Technology, 1:1,000), p-eIF2a (3398, Cell Signaling Technology, 1:1,000) and ubiquitin (3936, Cell Signaling Technology, 1:1,000)

N., Martin, D