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However, when the adaptive capacity is overcome, hemolytic erythrocyte-derived products act as signaling molecules, namely DAMPs, which promote inflammation via triggering TLR4-dependent activation of endothelial cells associated with NF-B-mediated synthesis of proinflammatory cytokines, TLR4-independent, ROS-, NADPH oxidase- and heme iron-mediated promotion of neutrophil extracellular traps (NETs) formation, TLR4-dependent activation of macrophages accompanied by secretion of tumor necrosis factor (TNF-), and MyD88/TRIF pathway-mediated TLR4-dependent microglia activation that triggers neuroinflammation [224]

Chem Soc Rev 50(1):556568

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Glycyl-L-histidyl-L-lysine complexed with copper (GHK-Cu) is an endogenous peptide with regenerative and anti-inflammatory properties that declines with age

Larger formats are available, but the 10mL size minimizes waste and improves protocol consistency for most labs

SRD5A2 protein was expressed in the insect Sf9 cells using the Bac-to-Bac baculovirus system (ThermoFisher)