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RNA extraction and RT-qPCR Total RNA was extracted from each sucrose gradient fraction using Trizol reagent (Invitrogen)

Nature 605, 747753

P426, P1126 Karasevska T

Ann Thorac Surg 113(6):1835-1844, 2022

Regarding liver and kidney damage in MTX treatment, Bedoui stated that MTX hepatotoxicity may occur due to the depletion of folate reserves and MTX-polyglutamate formation in the liver (Reference Bedoui, Guillot and Slambarom41)

Increase in the protein-bound form of glutathione in human blood after oral administration of glutathione
