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PMID 35570246

Meanwhile, less Cu 2+ is reduced to Cu + and then pumped into cells by CTR1, which removes the inhibition effect of Cu + on -secretase activity and promotes the generation of A

Additionally, OCR was detected using a commercial kit (600800, Cayman Chemical, Ann Arbor, MI, USA) following the manufacturers protocols

A result of decades of groundbreaking research, Dr

Yamagata, K

The samples were centrifuged, and the supernatant was subjected to a GSH assay based on a kinetic enzymatic recycling method that detects the oxidation of GSH by 5,5-dithiobis-2-nitrobenzoic acid (DTNB) and glutathione reductase to measure the GSH content in cells 74