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The combined application of ferroptosis inducers with traditional cancer treatments yields complex outcomes

When extracellular thiamine concentrations are below 2 mmol/L, active transport via THTR2 is possible

To ensure equal seeding density across wells, CFU validation was performed by plating aliquots on Middlebrook 7H11 agar and enumerating colonies after a 4-week incubation at 37 C

In many cases yes

UVVis absorbance was measured using UVVis spectrophotometer (Lambda 35, Perkin Elmer) to ensure the absence of large NPs, which commonly show absorption at about 520 nm

Despite these limitations, our findings provide preliminary but meaningful insights into the potential role of PON1 in oxidative imbalance