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Membrane was incubated at room temperature in blocking solution (1% bovine serum albumin, 1% goat serum in 1PBS) for 1 h, followed by incubation in blocking solution containing appropriate dilution of primary antibody for growth hormone receptor (R&D System, MN, USA) or phospho-Jak2 (Tyr1007/1008, Cell Signaling Technology, Inc

The treatment will vary depending on the needs of the patient and how much thinning of the hair has taken place

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Researchers designing combination protocols typically run controls with each compound in isolation alongside the combination arms to distinguish additive from synergistic effects and to identify which compound is driving each observed outcome