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Front Immunol 14:1277161 Feldman C, Anderson R (2021) The role of co-infections and secondary infections in patients with COVID-19

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Cells were resuspended in 5 to 10 mL of lysis buffer (20 mM sodium phosphate pH 7.5, 200 mM NaCl, 0.05% (w/v) Tween-20, 2 mM MgCl 2 , 0.5 mM TCEP, +400 U DNAse I, 12.5 mM imidazole and a tablet of Complete EDTA-free protease inhibitor cocktail, Roche, per 100 mL of buffer) per gram of cells

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