how to measure reduced glutathione Ratio of oxidized glutathione (GSH/GSSG). Data The optimised method of HPLC

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Then, 96-well plates were read in a fluorescence plate reader (Molecular Devices, SpectraMax Gemini XPS) in an excitation wavelength of 544 nm and an emission wavelength of 590 nm

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Plant Sci 165:13631367 Iturbe-Ormaetxe I, Moran JF, Arrese-Igor C, Gogorcena Y, Klucas RV, Becana M (1995) Activated oxygen and antioxidant defences in iron-deficient pea plants