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In the last few years, nanomaterial-based signal enhancement, multi-labelling, novel biosensor concepts, and smartphone-based point-of-care detection have provided an impetus to the advancement of next-generation diagnostic assays, whereas the current trend also unravels the wider applicability of robust biorecognition molecules far from conventional antibodies, like aptamers, affirmers, etc., to boost bioanalytical performance [1147]

Here follows a selection of 4 commonly used methods

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