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Description
2018 (11) CD010060
* P 110 13 genome copies/mL, Brain VTA) was injected using a glass micropipette connected to an ultra-micro injection pump (Nanoliter 2010, World Precision Instruments) with a flow rate of 70 nL/min
doi: 10.3389/fendo.2025.1551126 Received 24 December 2024 Accepted 31 March 2025 Published 17 April 2025 Volume 16 - 2025 Edited by Michio Kitajima, Takagi Hospital, Japan Reviewed by Long Bai, Zhejiang University, China Kequan Lin, Zhejiang University, China Updates Copyright 2025 Han, Xu, Yuan, Wen, Yang, Han and Zhang
Group K functioned as the control group, receiving only the basal diet
coli cells (100 mL) were collected (6000 rpm, 4 C, 10 min) and resuspended in 5 mL of lysis buffer containing 8 M urea, 4 % (w/v) CHAPS, and 1 % (w/v) DTT and sonicated on ice for 90 cycles (a working period of 3 s in a 6 s interval for each cycle) at a power output of 200 W by an ultrasonic disruptor (JY92-II, Scientz Biotechnology Co., Ningbo, China)
10.1016/j.intimp.2018.10.020 Int
