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As well as causing B12 deficiency, an increase in methylmalonic acid occurs, which is thought to result in loss of myelin in the peripheral nerves and, consequently, neuropathy, which may be symptomatic as early as 2 years into metformin treatment

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The Cu(II) chromophore the copper-2 ion bound at the histidine-imidazole chelation pocket of the GHK tripeptide absorbs visible light in the orange-red wavelength range and transmits the complementary blue

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So, if a total B12 blood test is under 148 pmol/L (200 ng/L), then this confirms deficiency and FYI they will be symptomatic at this level, albeit the forms of symptoms and severity varies

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