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After incubation with a streptavidin enzyme conjugate, the complex was visualized by color development with DAB (ZSGB Biological, China), and the slides were counterstained with hematoxylin

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In addition, results from the Danish National Birth Cohort (DNBC) [22] were also in disagreement with independently conducted work, with approximately 10% using APAP during the first 18 months of life [23] versus 65% using it within a 3-month period in an independent study evaluating a subset of the population assessed by the DNBC [24]