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Description
These variants display differences in their expression profiles, N- or C-terminal ends, as well as the number of transmembrane structural domains [34]
The filtered supernatant was run over a protein A/G column (Pierce), followed by extensive washing with PBS and the antibodies were eluted with 18 mL 0.1 M glycine pH 2.5 directly captured in 2 mL neutralization buffer (1 M TRIS pH 8.7)

Fasting and gut disorders: potential benefits outside of flare-ups [1:18:45]
The same thing is happening in the minimalist footwear movement

Stability of low-concentration ceftazidime in 0.9% sodium chloride injection and balanced salt solutions in plastic syringes under various storage conditions

Nelson et al., 2003