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.,LiuDXuYp53, oxidative stress, and aging.2011;15(6):16691678
10.1021/tx00031a001 Chem Res Toxicol
10.1016/s0741-8329(03)00104-6 11 ChenK.GuoN.ZhangR.WeiC.GuoR
Methylation of the DNA itself turns on specific genes
The optimal number of clusters was determined using the Silhouette method, and clusters were based on a period of 112 months
Pellets were thawed on ice for 1 h and lysed in 200 l 1% SDS in PBS supplemented with 1 protease inhibitors (cOmplete, EDTA-free Protease Inhibitor Cocktail, Roche, 11836153001) and shaken for 30 min at room temperature with 1:1,000 v/v benzonase nuclease (Sigma, E1014)
