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Afterward, 20 L of different concentrations of CF (ranging from 112.5 to 900 L/mL) and CA (15 to 120 L/mL), either in solution, liposomal form, or combination formulations, were added to the wells and incubated for 24 hours

This is done using different standard assays such as the DTNB assay, also known as Ellmans reagent (5,5-dithiobis (2-nitrobenzoic acid))

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