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Description
The GST cDNA sequence from pGEX-6P-2 (GE Healthcare) was PCR amplified with primers containing NcoI and KpnI restriction sites at the 5' and 3' extremities, respectively

GSH depletion triggers ferroptosis by inactivating GPX4 and promoting the accumulation of cytotoxic lipid peroxides 10
elevated levels may indicate increased oxidative demand

however, concluding the reasons for the observed changes is a complex task

Behav Brain Funct 6:32

Randomised Phase 2 study of maintenance linsitinib (OSI-906) in combination with erlotinib compared with placebo plus erlotinib after platinum-based chemotherapy in patients with advanced non-small cell lung cancer