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For the Sprague Dawley study, rats were single housed and had ad libitum access to a chow diet, kaolin pellets (K50001, Research Diets) and tap water

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This cycling approach allows the body to benefit from the peptide repair signaling during the active phase and then consolidate those gains during the off phase using its own endogenous repair mechanisms

Then, membranes were incubated for 2 hours using primary antibodies for CYP 11A1, 21A2, and CYP 19 using the dilution of 1:1000 and then washed twice using Tween-TBS (0.2 ml Tween/ liter TBS) for 20 min, then with TBS for 15 min

Hoang, T

The glutathione redox ratio (GSH: GSSG) is an indicator of overall glutathione status and intracellular reducing environment