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Threshold cycle numbers were transformed using the cycle threshold and relative value method described by the manufacturer and were expressed relative to glyceraldehyde-3-phosphate dehydrogenase, which was used as a housekeeping gene

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Potential side effects include: Nausea or mild stomach upset Fatigue Headache Gas or changes in bowel movements Low blood sugar (rare) If side effects are prolonged or difficult to tolerate, providers may recommend alternatives such as tirzepatide, which can have a different side effect profile.