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Founding Diplomat of the American Board of Holistic Medicine, December 2000

The most recent improvement to the method for generating amber codon mutants was the development of the plasmid pEVOL (Young et al., 2010)

Recently it was shown that dietary MR in mice leads to a rapid decrease in hepatic methionine, leading to inhibition of the translation initiation ternary complex, thereby slowing down protein translation

The fragments were integrated either into pSL4 (generating C-terminal 10xHis fusions) or into pDS94 (generating C-terminal GFP-10xHis fusions) (Plasmid list in Supplementary Table 4)

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