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These findings suggested that busulfan can significantly induce ROS creation and reduce antioxidants, leading to oxidative stress and lipid peroxidation in testicular tissue, which, in turn, can result in germ cell death, spermatogenesis impairment, and infertility (49)

Cell culture and treatments HepG2 (RRID: CVCL_0027) cells and C2C12 (RRID: CVCL_0188) cells were purchased from Shanghai QuiCell Biotechnology Co., Ltd

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NGFRs nerve growth factor receptors acetyl L-carnitine is clinically used to treat depression associated with the primary degenerative disease (Alzheimer's Dementia), is also prescribed to treat the secondary degenerative disease (vascular dementia), and is a broad-spectrum agent which can be used as a first-selective drug ex hibiting excellent ameliorating effects on early- stage dementia patients (The Journal of Applied Pharmacology (2001) 9:285-290)