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Generation of monoclonal HEK293T knockout cell lines Semiconfluent HEK293T cells in 6-well plates were transfected with 2.5g of a pSpCas9(BB)-2A-EGFP plasmid containing either DCAF16 or FBXO22 sgRNA using Lipofectamine 2000 (Thermo Fisher Scientific, 11668027)

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