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glutathione glycolysis Overview of glycolysis, PPP, metabolism and recycling, and Full article: The anti-oxidant capacity
Description
Membranes were blocked with 5% (w/v) milk powder in PBS for 1 h and incubated for 16 h at 4 C with one of the following primary antibodies: rabbit anti-SLC31A1/CTR1 (Abcam, Cambridge, UK), mouse anti-GAPDH (Santa Cruz Biotechnology, Dallas, TX, USA), mouse anti-XPF (Thermo Fisher, Waltham, MA, USA), mouse anti-ERCC1 (Santa Cruz Biotechnology), mouse anti-Tubulin (Abcam), rabbit anti-NRF2 (Abcam)

The light (~28 kDa), or modulatory subunit alters, or regulates, the activity of the holoenzyme by reducing the Km for glutamate and elevating the Ki for GSH, thereby making the enzyme more efficient and less sensitive to feedback inhibition
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