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At the end of the incubation of 30 min or 90 min, some of the MCTS were transferred to a new agarose-coated 96-well plate, and cultured in drug-free medium for different lengths of time (2, 6, 12, and 24 h) to further study the post treatment effects and drug elimination

USP22 protects against myocardial ischemia-reperfusion injury via the SIRT1-p53/SLC7A11-dependent inhibition of ferroptosis-induced cardiomyocyte death

Between 2 and 12% of patients receiving high-dose MTX therapy may get acute kidney injury, mostly as a result of crystal nephropathy caused by MTX and its metabolite, 7-OH-MTX

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