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Cellular-level transcriptomic analysis indicated that it may exert these effects through pathways such as the PPAR signaling pathway, oxidative phosphorylation, and ribosome-related processes [84]

However, a critical appraisal of this evidence is imperative, particularly for distinguishing robust, specific mechanisms from potential artifacts associated with PAINS prevalent in its phytochemical profile ( in vitro findings requires careful evaluation alongside in vivo and clinical data to establish a mechanistic foundation for evidence-based clinical implementation

ZaeemzadehNJahanian SadatmahallehSZiaeiSKazemnejadAMovahedinejadMMottaghiAet al

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These include the Sperm Chromatin Structural Assay (SCSA), Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL), Comet, and Sperm Chromatin Dispersion (SCD) assays [49]

At the same time, studies which evaluated a profile aimed at neuroinflammation suggest ALA doses of 100 to 200 mg/kg to inhibit inflammatory pathways in the central nervous system, such as the mitogen activated protein kinase (MAPK) signaling pathway, related to the ERK, JNK and NF-kB pathways