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100 nuclei from more than 20 animals were examined for each group ( e,h )

This is because there is a mutation or abnormality in the relevant gene: COL1A2 for cvEDS, and COL1A1 and COL3A1 for vEDS (no gene has been identified yet for hEDS)

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I felt aches and agitation that woke me up, but my wife slept it off more

(32) The lag-time until increasing fluorescence was assessed by visual inspection of the fluorescence versus time plots for each sample

That's why you've been taking ibuprofen for months without seeing lasting improvement