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Real-time polymerase chain reaction (PCR) was performed with Takyon Rox SYBR master mix (Eurogentec, Seraing, Belgium) in a StepOnePlus (Applied Biosystems, Life Technologies, Foster City, CA, USA) using the following primers: IL-6 F 5ACTGGCAGAAAATAAGCTGAATCTTC 3 and R 5TGATCAAGCAAATCGCCTGAT3

Fang B, Roth JA

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Importantly, co-treatment with KI696 was able to sensitize KEAP1 wild type cancer cells that were relatively insensitive to CB-839 (Figure 5B and Figure 5figure supplement 1)

The 19-kDa antigen of Mycobacterium tuberculosis is a major adhesin that binds the mannose receptor of THP-1 monocytic cells and promotes phagocytosis of mycobacteria
