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Tetrameric assembly and conservation in the ATP-binding domain of rat branched-chain -ketoacid dehydrogenase kinase

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Prepared cDNA was used in the qPCR analyzer (StepOne, Applied Biosystems, Singapore) using MAXIMA SYBR Green qPCR Master Mix with the following program: 1 cycle at 95 C for 10 min

Compared with groups A1, 2 and 3, the Johnsen scores were decreased in groups B, C and D, and the difference was statistically significant ( P 0.05)

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