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One sure way to know if your body is deficient in B12 would be having a simple blood test done, but that is not always necessary

Complications of Left Untreated Untreated pernicious anemia can lead to severe complications such as: Neurological Damage: Permanent nerve damage and cognitive impairments

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Do not shake or vortex aggressively, since shearing forces can damage peptide bonds

Yes, in research

It is commonly used in research involving enzymatic pathway modulation and advanced biochemical systems