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(23) found that knocking down NCOA4 gene, inhibiting ferritinophagy, and increasing iron storage could inhibit ferroptosis induced by smoke exposure in chronic obstructive pulmonary disease

When cells were 3050% confluent in 6-well culture plates, the medium was replaced with fresh one 30 min before transfection

doi: 10.1017/S0033291717003336 22 FerrariA

AI energized hydrogel design, optimization and application in biomedicine

In addition, there are other neuropathological mechanisms induced by L-Glu, including excitotoxicity, in which L-Glu overactivates its receptors, including those for N-methyl-D-aspartate (NMDA), -amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA), kainate acid (KA), and metabotropic glutamate (mGlu), resulting in an excessive influx of calcium ions (Ca 2+ ) into neurons, leading to the generation of reactive radicals and increased levels of proapoptotic proteins [29,30,31,32]
Astrocyte-secreted GDNF and glutathione antioxidant system protect neurons against 6OHDA cytotoxicity
